RSK3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RSK3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14446-1-AP

Synonyme

HU 2, MAPKAP kinase 1c, MAPKAPK 1c, MAPKAPK1C, p90 RSK 2, p90 RSK3, p90RSK2, pp90RSK3, Ribosomal S6 kinase 3, RPS6KA2, RSK, RSK 3, RSK3, S6K alpha, S6K alpha 2, S6K alpha2

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 14446-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14446-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14446-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14446-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14446-1-AP

  IP Result of anti-RSK3 (IP:14446-1-AP, 4ug; Detection:14446-1-AP 1:800) with HeLa cells lysate 4000ug.

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  IHC staining of human lung cancer using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14446-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14446-1-AP (RSK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14446-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA RSK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSK3 fusion protein Ag5862
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 2
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC002363
• Gene symbol: RPS6KA2
• Gene ID (NCBI): 6196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RSK3, also named as RPS6KA2, belongs to the protein kinase superfamily. RSK3 is a serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. In addition RSK3 may function as tumor suppressor in epithelial ovarian cancer cells(Uniprot). The molecular weight of RSK3 is 83 kDa.

Publikationen

Species	Application	Title
human	WB	Theranostics; Kartogenin hydrolysis product 4-aminobiphenyl distributes to cartilage and mediates cartilage regeneration.; Authors - Shuai Zhang
mouse	WB,IHC	Theranostics; RSK-3 promotes cartilage regeneration via interacting with rpS6 in cartilage stem/progenitor cells.; Authors - Shuai Zhang ; KD Validated
human	IHC	Epigenomics; Multiomics analysis identifies key genes and pathways related to N6-methyladenosine RNA modification in ovarian cancer.; Authors - Haoya Xu
human	CoIP	Cell Death Discov; MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway.; Authors - Xiaolin Guan
human	WB	J Med Chem; Discovery, Optimization, and Structure-Activity Relationship Study of Novel and Potent RSK4 Inhibitors as Promising Agents for the Treatment of Esophageal Squamous Cell Carcinoma; Authors - Yuan Yuan