RPS24 Polyklonaler Antikörper

RPS24 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14831-1-AP

Synonyme

40S ribosomal protein S24, DBA3, ribosomal protein S24, RPS24

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 14831-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14831-1-AP (RPS24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14831-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14831-1-AP (RPS24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14831-1-AP (RPS24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 14831-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 14831-1-AP (RPS24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

14831-1-AP bindet in WB, IHC, IF, ELISA RPS24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS24 fusion protein Ag6621
• Vollständiger Name: ribosomal protein S24
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC000523
• Gene symbol: RPS24
• Gene ID (NCBI): 6229
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

40S ribosomal protein S24 (RPS24) is a 133 amino acid protein, which belongs to the ribosomal protein S24e family. RPS24 is required for processing of pre-rRNA and maturation of 40S ribosomal subunits. RPS24 has an association with Diamond-Blackfan anemia 3.

Publikationen

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; ZEA and DON inhibited inflammation after L. monocytogenes infection and induced ribosomal hyperfunction.; Authors - Guodong Cai
human	IHC	Int J Mol Sci; RPS24 Is Associated with a Poor Prognosis and Immune Infiltration in Hepatocellular Carcinoma; Authors - Haiyuan Li
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao