RPS21 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPS21 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16946-1-AP

Synonyme

40S ribosomal protein S21, ribosomal protein S21, RPS21

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Galerie der Validierungsdaten

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  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16946-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 16946-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16946-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16946-1-AP (RPS21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, HepG2-Zellen, Rattenlebergewebe, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

16946-1-AP bindet in WB, IHC, IF, ELISA RPS21 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS21 fusion protein Ag10557
• Vollständiger Name: ribosomal protein S21
• Berechnetes Molekulargewicht: 81 aa, 9 kDa
• Beobachtetes Molekulargewicht: 9 kDa
• GenBank-Zugangsnummer: BC018140
• Gene symbol: RPS21
• Gene ID (NCBI): 6227
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit.

Publikationen

Species	Application	Title
human	IHC	Oncol Lett; Identification of the key genes and pathways in prostate cancer.; Authors - Shutong Fan
human	WB,IHC	Med Oncol; Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21.; Authors - Zumu Liang ; KD Validated
mouse	IHC	Reprod Sci; Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes; Authors - Natsuki Nakanishi