RPS14 Polyklonaler Antikörper

RPS14 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 16683-1-AP

Synonyme

40S ribosomal protein S14, EMTB, ribosomal protein S14, RPS14

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Galerie der Validierungsdaten

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  WB analysis using 16683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16683-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16683-1-AP (RPS14 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16683-1-AP (RPS14 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 16683-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPS14 antibody 16683-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of U2OS cells using 16683-1-AP

  IF result of 16683-1-AP (anti-RPS14) in U2OS cell by Dr. Nancy Kedersha. (cytosolic small ribosomal and nucleolus; Green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

16683-1-AP bindet in WB, IHC, IF, ELISA RPS14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, arabidopsis, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS14 fusion protein Ag10012
• Vollständiger Name: ribosomal protein S14
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC006784
• Gene symbol: RPS14
• Gene ID (NCBI): 6208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].

Publikationen

Species	Application	Title
human	IF	Nucleic Acids Res; Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.; Authors - Fujimura Ken K
human	WB	Acta Neuropathol Commun; Altered ribosomal function and protein synthesis caused by tau.; Authors - Harrison Tudor Evans
Arabidopsis	WB	J Exp Bot; The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis.; Authors - Ilyeong Choi
human	WB	Free Radic Biol Med; Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.; Authors - Reiko Iida
	WB	J Exp Bot; Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.; Authors - Yanyun Shi
zebrafish	IF	Blood Adv; TLR7 ligation augments hematopoiesis in Rps14 (uS11) deficiency via paradoxical suppression of inflammatory signaling.; Authors - Oscar A Peña