RPS13 Polyklonaler Antikörper

RPS13 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16680-1-AP

Synonyme

40S ribosomal protein S13, ribosomal protein S13, RPS13

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Galerie der Validierungsdaten

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  WB analysis using 16680-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16680-1-AP (RPS13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 16680-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16680-1-AP (RPS13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16680-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16680-1-AP (RPS13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16680-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16680-1-AP (RPS13 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, mouse oterus tissue, rat oterus tissue
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

16680-1-AP bindet in WB, IHC, IF, ELISA RPS13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS13 fusion protein Ag10008
• Vollständiger Name: ribosomal protein S13
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 14 kDa, 17 kDa
• GenBank-Zugangsnummer: BC006772
• Gene symbol: RPS13
• Gene ID (NCBI): 6207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS13, also named as 40S ribosomal protein S13, belongs to the ribosomal protein S15P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. RPS13 is located in the cytoplasm. It is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Publikationen

Species	Application	Title
	WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
human	WB	Stem Cells; Genome-wide studies reveal that Lin28 enhances the translation of genes important for growth and survival of human embryonic stem cells.; Authors - Peng Shuping S
human	WB	N/A; N/A; Authors - N/A
	WB	Cell; An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes; Authors - Keely Oltion