RPL3 Monoklonaler Antikörper

RPL3 Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2G1B8

Kat-Nr. : 66130-1-Ig

Synonyme

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 66130-1-Ig

  Western blot analysis of RPL3 in various cell lines using Proteintech antibody 66130-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66130-1-Ig

  Western blot analysis of RPL3 in various cell lines and tissues using Proteintech antibody 66130-1-Ig at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66130-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66130-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66130-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66130-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66130-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66130-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66130-1-Ig (RPL3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66130-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66130-1-Ig (RPL3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

66130-1-Ig bindet in WB, IHC, IF, ELISA RPL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPL3 fusion protein Ag20400
• Vollständiger Name: ribosomal protein L3
• Berechnetes Molekulargewicht: 46/27 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC012786
• Gene symbol: RPL3
• Gene ID (NCBI): 6122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Publikationen

Species	Application	Title
human	IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Nat Commun; Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.; Authors - Hauke S Hillen
human	WB	Elife; METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.; Authors - Eriko Matsuura-Suzuki
mouse	IF	J Biol Chem; Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis.; Authors - Matthew J Brody