RPL28 Polyklonaler Antikörper

RPL28 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 16649-1-AP

Synonyme

60S ribosomal protein L28, ribosomal protein L28, RPL28

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 16649-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16649-1-AP (RPL28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16649-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16649-1-AP (RPL28 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16649-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL28 antibody 16649-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

16649-1-AP bindet in WB, IF, FC, ELISA RPL28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL28 fusion protein Ag10036
• Vollständiger Name: ribosomal protein L28
• Berechnetes Molekulargewicht: 137 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC010173
• Gene symbol: RPL28
• Gene ID (NCBI): 6158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Genes Dev; Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.; Authors - Andrew J Finch
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	WB	Aging Cell; Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence.; Authors - Asimina Pantazi
mouse	WB	Elife; Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts; Authors - Jasbir Singh Dalal
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
		Sci Rep; Germline variability and tumor expression level of ribosomal protein gene RPL28 are associated with survival of metastatic colorectal cancer patients.; Authors - Adrien Labriet