RPL23A Polyklonaler Antikörper

RPL23A Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16386-1-AP

Synonyme

60S ribosomal protein L23a, MDA20, ribosomal protein L23a, RPL23A

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Galerie der Validierungsdaten

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  WB analysis using 16386-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16386-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16386-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16386-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16386-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16386-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16386-1-AP

  IP Result of anti-RPL23A (IP:16386-1-AP, 4ug; Detection:16386-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human liver using 16386-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16386-1-AP (RPL23A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16386-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16386-1-AP (RPL23A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16386-1-AP

  Immunofluorescent analysis of HepG2 cells using 16386-1-AP (RPL23A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Lebergewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• RIP: See 1 publications below

Produktinformation

16386-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA RPL23A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL23A fusion protein Ag9424
• Vollständiger Name: ribosomal protein L23a
• Berechnetes Molekulargewicht: 156 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18-23 kDa
• GenBank-Zugangsnummer: BC014459
• Gene symbol: RPL23A
• Gene ID (NCBI): 6147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
mouse	WB,RIP	Nat Commun; The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis.; Authors - Mehdi Pirouz
human	WB	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič
mouse	WB	Gene; Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.; Authors - Trieu-Duc Vu
human	WB	Cell Death Dis; HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer.; Authors - Zhiyuan Zhang