RPL17 Monoklonaler Antikörper

RPL17 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1C5E6

Kat-Nr. : 67223-1-Ig

Synonyme

60S ribosomal protein L17, 60S ribosomal protein L23, FLJ92089, PD 1, ribosomal protein L17, RPL17, rpL23

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Galerie der Validierungsdaten

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  WB analysis using 67223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67223-1-Ig (RPL17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67223-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67223-1-Ig (RPL17 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

67223-1-Ig bindet in WB, IHC, IF, ELISA RPL17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPL17 fusion protein Ag5393
• Vollständiger Name: ribosomal protein L17
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC066323
• Gene symbol: RPL17
• Gene ID (NCBI): 6139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.

Publikationen

Species	Application	Title
rat	WB,IHC	J Orthop Translat; Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression.; Authors - Zhenyuan Wei
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk