RPL17 Polyklonaler Antikörper

RPL17 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14121-1-AP

Synonyme

60S ribosomal protein L17, 60S ribosomal protein L23, PD 1, ribosomal protein L17, RPL17, rpL23, uL22

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Galerie der Validierungsdaten

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  WB analysis using 14121-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14121-1-AP (RPL17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14121-1-AP

  IP result of anti-RPL17(IP:14121-1-AP, 4ug; Detection:14121-1-AP 1:2000) with HeLa cells lysate 1080 ug.

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  IHC staining of human pancreas cancer using 14121-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14121-1-AP (RPL17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

14121-1-AP bindet in WB, IP, IHC, IF, ELISA RPL17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL17 fusion protein Ag5270
• Vollständiger Name: ribosomal protein L17
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC066323
• Gene symbol: RPL17
• Gene ID (NCBI): 6139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.; Authors - Martin Gamerdinger
mouse	WB	Nat Chem Biol; Identifying the functional contribution of the defatty-acylase activity of SIRT6.; Authors - Xiaoyu Zhang
human	WB	Elife; METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.; Authors - Eriko Matsuura-Suzuki
mouse	WB	Front Endocrinol (Lausanne); GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging.; Authors - Bronwen Martin
	IF	Cell Rep; On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.; Authors - Christine E. Holt
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren