RPL14 Polyklonaler Antikörper

RPL14 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14991-1-AP

Synonyme

60S ribosomal protein L14, CAG ISL 7, CTG B33, hRL14, L14, ribosomal protein L14, RL14, RPL14

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Galerie der Validierungsdaten

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  WB analysis using 14991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14991-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14991-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14991-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 14991-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14991-1-AP

  IP Result of anti-RPL14 (IP:14991-1-AP, 4ug; Detection:14991-1-AP 1:1000) with mouse liver tissue lysate 6800ug.

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14991-1-AP (RPL14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14991-1-AP (RPL14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14991-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL14 antibody 14991-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14991-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA RPL14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL14 fusion protein Ag6976
• Vollständiger Name: ribosomal protein L14
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC000606
• Gene symbol: RPL14
• Gene ID (NCBI): 9045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

Publikationen

Species	Application	Title
mouse	IF	Cell Res; Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.; Authors - Xiaochen Gai
human	WB,IF	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB	Am J Cancer Res; UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc; Authors - Dalian Gong
human	CoIP,WB	Cell Discov; The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination; Authors - Duo Wu