RPL11 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPL11 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

30

Rezensionen (2)

Kat-Nr. : 16277-1-AP

Synonyme

60S ribosomal protein L11, CLL associated antigen KW 12, DBA7, GIG34, ribosomal protein L11, RPL11

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Galerie der Validierungsdaten

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  WB analysis of human liver using 16277-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 16277-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 16277-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16277-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16277-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16277-1-AP (RPL11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16277-1-AP (RPL11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 16277-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 16277-1-AP (RPL11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, BxPC-3-Zellen, Mauslungengewebe, Maus-Pankreasgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 27 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16277-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA RPL11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL11 fusion protein Ag9356
• Vollständiger Name: ribosomal protein L11
• Berechnetes Molekulargewicht: 177 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC018970
• Gene symbol: RPL11
• Gene ID (NCBI): 6135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao
mouse	IF	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
human	WB	Am J Hum Genet; Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer.; Authors - Koji Nakamura
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Blood; Defective ribosome assembly in Shwachman-Diamond syndrome.; Authors - Wong Chi C CC
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anita (Verified Customer) (12-22-2020)

Works for C. elegans at a protein concentration of 5 micrograms and above

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans

RPL11 Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:16277-1-AP)

FH

Zehua (Verified Customer) (12-17-2019)

It works excellent for WB analysis. I strongly suggested this antibody.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cell lines

RPL11 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in Cell lines (Cat no:16277-1-AP)

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