RPAP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPAP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 17401-1-AP

Synonyme

C1orf82, RPAP2

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Galerie der Validierungsdaten

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  WB analysis using 17401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17401-1-AP (RPAP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17401-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17401-1-AP (RPAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17401-1-AP

  IP Result of anti-RPAP2 (IP:17401-1-AP, 4ug; Detection:17401-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of mouse brain using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 17401-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RPAP2 antibody (17401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, MOLT-4-Zellen, THP-1-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maus-Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

17401-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA RPAP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPAP2 fusion protein Ag11374
• Vollständiger Name: RNA polymerase II associated protein 2
• Berechnetes Molekulargewicht: 612 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC039014
• Gene symbol: RPAP2
• Gene ID (NCBI): 79871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPAP2 belongs to the RNA Pol II-associated protein (RPAP) family, a set of proteins involved in human Pol II machinery. Recently it has been reported that RPAP2 is required for efficient transcription of snRNA genes by Pol II. siRNA-mediated knockdown of RPAP2 caused defects in snRNA gene expression. (22137580)

Publikationen

Species	Application	Title
human	WB	Mol Cell; Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes.; Authors - Egloff Sylvain S
human	WB	Mol Cell; Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate.; Authors - Tsun-Kai Chang ; KD Validated
human	WB	Nat Struct Mol Biol; RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation.; Authors - Zuyao Ni ; KD Validated
human	WB	Nucleic Acids Res; Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.; Authors - Forget Diane D
human	WB	PLoS One; Herpes Simplex Virus 1 (HSV-1) ICP22 Protein Directly Interacts with Cyclin-Dependent Kinase (CDK)9 to Inhibit RNA Polymerase II Transcription Elongation.; Authors - Justyna Zaborowska
human	WB, IF	Histopathology; Abnormal expression of RNA polymerase II associated proteins in muscle of patients with myofibrillar myopathies.; Authors - Valeria Guglielmi