RPAIN Monoklonaler Antikörper

RPAIN Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D8E3

Kat-Nr. : 68546-1-Ig

Synonyme

HRIP, RIP, RPA interacting protein, RPAIN

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Galerie der Validierungsdaten

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  WB analysis using 68546-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68546-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 68546-1-Ig

  A2780 cells were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A2780-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68546-1-Ig bindet in WB, ELISA RPAIN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPAIN fusion protein Ag7844
• Vollständiger Name: RPA interacting protein
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC004451
• Gene symbol: RPAIN
• Gene ID (NCBI): 84268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. RPAIN was originally identified as a nuclear transporter of RPA in Xenopus (PMID: 23010595). The human RIPα gene encodes several splice isoforms, of which hRIPα and hRIPβ are the major translation products in vivo.hRIPα transports RPA into the nucleus by shuttling between the cytoplasm and nucleus while hRIPβ is localized in promyelocytic leukemia (PML) nuclear bodies.