ROR2 Polyklonaler Antikörper

ROR2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 25842-1-AP

Synonyme

BDB, BDB1, NTRKR2, ROR2

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Galerie der Validierungsdaten

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  WB analysis using 25842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25842-1-AP (ROR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25842-1-AP (ROR2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25842-1-AP bindet in WB, ELISA ROR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ROR2 fusion protein Ag22959
• Vollständiger Name: receptor tyrosine kinase-like orphan receptor 2
• Berechnetes Molekulargewicht: 943 aa, 105 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC130522
• Gene symbol: ROR2
• Gene ID (NCBI): 4920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tyrosine-protein kinase transmembrane receptor ROR2 belongs to the ROR-family of receptor tyrosine kinases (RTKs) (PMID: 12815588). ROR2 is a developmentally regulated protein that has significant expression and roles in a wide variety of tissues during early embryonic development. It was established that ROR2 plays a key role in skeletal and neural organogenesis, as well as in midgut development in the mouse, but then becomes repressed in adult tissues. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PMID: 25614331). Mutations in ROR2 gene are associated with autosomal recessive Robinow syndrome and autosomal dominant brachydactyly type B (PMID: 12815588).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Stephen (Verified Customer) (08-02-2022)

IP performed using in HeLa cells in RIPA buffer using 5ug of ror2 antibody per 1ug/ml of protein lysate. pulldown performed using magnetic protein A beads (NEB). western blot probed with another ror2 specific antibody raised in mouse ( MABS1152 )

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 5ug of protein used for IP
• Cell Tissue Type: HeLa

ROR2 Antibody Immunoprecipitation validation (5ug of protein used for IP dilution) in HeLa (Cat no:25842-1-AP)