ROGDI Polyklonaler Antikörper

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• KD/KO Validated

ROGDI Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17047-1-AP

Synonyme

Protein rogdi homolog, ROGDI, rogdi homolog (Drosophila)

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Galerie der Validierungsdaten

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  WB analysis of human brain using 17047-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17047-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17047-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17047-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17047-1-AP

  IP Result of anti-ROGDI (IP:17047-1-AP, 4ug; Detection:17047-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Nierengewebe, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 3 publications below

Produktinformation

17047-1-AP bindet in WB, IP, IHC, IF,ELISA ROGDI und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, drosophila, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ROGDI fusion protein Ag10688
• Vollständiger Name: rogdi homolog (Drosophila)
• Berechnetes Molekulargewicht: 287 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC012901
• Gene symbol: ROGDI
• Gene ID (NCBI): 79641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROGDI encoded by the ROGDI gene, which encodes a leucine-zipper protein with high expression in the human spinal cord and brain, acts as a positive regulator of cell proliferation. Defections of ROGDI are the cause of Kohlschuetter-Toenz syndrome (KTZS)

Publikationen

Species	Application	Title
human	IF	Am J Hum Genet; Mutations in ROGDI Cause Kohlschütter-T?nz Syndrome.; Authors - Schossig Anna A
Drosophila	IF	Am J Hum Genet; A nonsense mutation in the human homolog of Drosophila rogdi causes Kohlschutter-Tonz syndrome.; Authors - Mory Adi A
rat	WB, IF	Sci Rep; The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein.; Authors - Donatus Riemann
human	WB	Cancer Biol Ther; Downregulation of a novel human gene, ROGDI, increases radiosensitivity in cervical cancer cells.; Authors - Yi-Fan Chen ; KD Validated