ROCK2-Specific(C-term) Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ROCK2-Specific(C-term) Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 20248-1-AP

Synonyme

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 20248-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20248-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20248-1-AP

  Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

20248-1-AP bindet in WB, IHC, IF, ELISA ROCK2-Specific(C-term) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Rho-associated, coiled-coil containing protein kinase 2
• Berechnetes Molekulargewicht: 161 kDa
• Beobachtetes Molekulargewicht: 164 kDa
• GenBank-Zugangsnummer: NM_004850
• Gene symbol: ROCK2
• Gene ID (NCBI): 9475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

Publikationen

Species	Application	Title
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB,IHC	Cell Prolif; The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells.; Authors - Wencan Ke
human	WB,IHC,IF	Exp Neurol; PMID: 27637803; Authors - Jiong Yue
human	WB	Chin J Nat Med; Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation; Authors - Suping Li
rat	IHC	Oxid Med Cell Longev; Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression.; Authors - Yunqiang He
mouse	WB	ASN Neuro; Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.; Authors - Carlos E Pedraza ; KD Validated