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ROBO2 Polyklonaler Antikörper

ROBO2 Polyklonal Antikörper für IHC, IP,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IP, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19986-1-AP

Synonyme

KIAA1568, ROBO2, Roundabout homolog 2, SAX3

Galerie der Validierungsdaten

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  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using ROBO2 Polyclonal antibody (19986-1-AP)

    IP experiment of SH-SY5Y using 19986-1-AP

    IP Result of anti-ROBO2 (IP:19986-1-AP, 4ug; Detection:19986-1-AP 1:300) with SH-SY5Y cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ROBO2 Polyclonal antibody (19986-1-AP)

    IHC staining of human cerebellum using 19986-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ROBO2 Polyclonal antibody (19986-1-AP)

    IHC staining of human cerebellum using 19986-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ROBO2 Polyclonal antibody (19986-1-AP)

    IHC staining of human prostate hyperplasia using 19986-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ROBO2 Polyclonal antibody (19986-1-AP)

    IHC staining of human prostate hyperplasia using 19986-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche IPSH-SY5Y-Zellen
Erfolgreiche Detektion in IHChumanes Cerebellum-Gewebe, humanes Prostatahyperplasie-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19986-1-AP bindet in IP, IHC,ELISA ROBO2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name roundabout, axon guidance receptor, homolog 2 (Drosophila)
Berechnetes Molekulargewicht 151 kDa
GenBank-ZugangsnummerNM_002942
Gene symbol ROBO2
Gene ID (NCBI) 6092
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROBO2, also named as KIAA1568, belongs to the immunoglobulin superfamily and ROBO family. ROBO2 is a receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Defects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2). A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. The antibody recognizes the C-term of ROBO.