ROBO1 Monoklonaler Antikörper

ROBO1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC

Konjugation

Unkonjugiert

CloneNo.

2H4H5

Kat-Nr. : 67922-1-Ig

Synonyme

Deleted in U twenty twenty, DUTT1, FLJ21882, H Robo 1, ROBO1, Roundabout homolog 1, SAX3

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 67922-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 67922-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67922-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67922-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67922-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 67922-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67922-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67922-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67922-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67922-1-Ig bindet in WB, IHC ROBO1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ROBO1 fusion protein Ag18074
• Vollständiger Name: roundabout, axon guidance receptor, homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1651 aa, 181 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC112336
• Gene symbol: ROBO1
• Gene ID (NCBI): 6091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.