RNF40 Polyklonaler Antikörper

RNF40 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 15621-1-AP

Synonyme

BRE1 B, BRE1B, KIAA0661, RBP95, ring finger protein 40, RNF40, STARING

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Galerie der Validierungsdaten

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  WB analysis of human brain using 15621-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15621-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15621-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 15621-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15621-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 15621-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15621-1-AP (RNF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Mauslebergewebe, Mauslungengewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15621-1-AP bindet in WB, IF,ELISA RNF40 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF40 fusion protein Ag8047
• Vollständiger Name: ring finger protein 40
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 114 kDa
• GenBank-Zugangsnummer: BC006133
• Gene symbol: RNF40
• Gene ID (NCBI): 9810
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (03-11-2022)

This antibody works well for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Colon cancer cells