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RNF212 Polyklonaler Antikörper

RNF212 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21178-1-AP

Synonyme

ring finger protein 212, RNF212, ZHP3

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human heart tissue using RNF212 Polyclonal antibody (21178-1-AP)

    WB analysis of human heart using 21178-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21178-1-AP (RNF212 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Herzgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21178-1-AP bindet in WB, ELISA RNF212 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RNF212 fusion protein Ag15457
Vollständiger Name ring finger protein 212
Berechnetes Molekulargewicht 297 aa, 33 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC050356
Gene symbol RNF212
Gene ID (NCBI) 285498
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF212(RING finger protein 212)acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. It may be a SUMO E3 ligase that mediates the sumoylation of target proteins MSH4 and MSH5, leading to enhanced binding to recombination sites.

Protokolle

Produktspezifische Protokolle
WB protocol for RNF212 antibody 21178-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

iru (Verified Customer) (07-14-2020)

I got a clean band but it is higher than the expected molecular weight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells
RNF212 Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:21178-1-AP)