RNF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNF2 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 16031-1-AP

Synonyme

BAP 1, BAP1, DING, HIP2 interacting protein 3, HIPI3, Protein DinG, RING finger protein 1B, ring finger protein 2, RING finger protein BAP 1, RING1B, RING2, RNF2

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 16031-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16031-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16031-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 16031-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16031-1-AP

  IP Result of anti-RNF2 (IP:16031-1-AP, 4ug; Detection:16031-1-AP 1:800) with K-562 cells lysate 1200ug.

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  IF Staining of HeLa using 16031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16031-1-AP (RNF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, humanes Hirngewebe, NIH/3T3-Zellen, U-937-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

16031-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA RNF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF2 fusion protein Ag8900
• Vollständiger Name: ring finger protein 2
• Berechnetes Molekulargewicht: 336 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC012583
• Gene symbol: RNF2
• Gene ID (NCBI): 6045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Coordinated regulation of polycomb group complexes through microRNAs in cancer.; Authors - Qi Cao ; KD Validated
human	IHC	Cancer Cell; The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.; Authors - Wenjing Su ; KD Validated
human	WB,IHC	FEBS J; Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy; Authors - Yan Wei
human	WB, IF	Front Cell Dev Biol; RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells.; Authors - Qi Yan ; KD Validated
human	WB	Front Oncol; Long non-coding RNA PRR7-AS1 promotes osteosarcoma progression via binding RNF2 to transcriptionally suppress MTUS1; Authors - Gu Chen-Xi ; KD Validated
human	WB,IHC,CoIP	Biochim Biophys Acta Mol Cell Res; RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4; Authors - Jiwei Wang