RNF168 Polyklonaler Antikörper

RNF168 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 21393-1-AP

Synonyme

ring finger protein 168, RNF168

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 21393-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21393-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21393-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 21393-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

• 

  IP experiment of HepG2 using 21393-1-AP

  IP Result of anti-RNF168 (IP:21393-1-AP, 4ug; Detection:21393-1-AP 1:500) with HepG2 cells lysate 2400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

21393-1-AP bindet in WB, IP, IF, CoIP, ELISA RNF168 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF168 fusion protein Ag16055
• Vollständiger Name: ring finger protein 168
• Berechnetes Molekulargewicht: 571 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC033791
• Gene symbol: RNF168
• Gene ID (NCBI): 165918
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IP	Nat Commun; Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.; Authors - Qinglei Hang
human		Nucleic Acids Res; GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.; Authors - Xiaopeng Lu
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	WB	EMBO Rep; An LTR retrotransposon-derived lncRNA interacts with RNF169 to promote homologous recombination.; Authors - Bing Deng
human	WB	FASEB J; RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage.; Authors - Xiaopeng Lu
human	IP	PLoS Pathog; p62-mediated Selective autophagy endows virus-transformed cells with insusceptibility to DNA damage under oxidative stress.; Authors - Ling Wang