- Featured Product
- KD/KO Validated
RIPK2 Polyklonaler Antikörper
RIPK2 Polyklonal Antikörper für IP, WB,ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IP, IF, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15366-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293T-Zellen, HeLa-Zellen, Maushirngewebe, THP-1-Zellen |
Erfolgreiche IP | HEK-293T-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 10 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Produktinformation
15366-1-AP bindet in WB, IP, IF, CoIP, ELISA RIPK2 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | RIPK2 fusion protein Ag0909 |
Vollständiger Name | receptor-interacting serine-threonine kinase 2 |
Berechnetes Molekulargewicht | 61 kDa |
Beobachtetes Molekulargewicht | 46-55 kDa, 61-72 kDa |
GenBank-Zugangsnummer | BC004553 |
Gene symbol | RIPK2 |
Gene ID (NCBI) | 8767 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for RIPK2 antibody 15366-1-AP | Protokoll herunterladen |
IP protocol for RIPK2 antibody 15366-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Antioxidants (Basel) M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide | ||
Obesity (Silver Spring) Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance. | ||
J Pharmacol Exp Ther Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.
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Front Cell Infect Microbiol Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways. | ||
Immunol Lett A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation. | ||
Front Genet Knockdown of RIPK2 Inhibits Proliferation and Migration, and Induces Apoptosis via the NF-κB Signaling Pathway in Gastric Cancer.
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