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  • KD/KO Validated

RIPK2 Polyklonaler Antikörper

RIPK2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15366-1-AP

Synonyme

CARD3, CARDIAK, CCK, GIG30, Receptor interacting protein 2, RICK, RIP 2, RIP2, RIPK2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293T-Zellen, HeLa-Zellen, Maushirngewebe, THP-1-Zellen
Erfolgreiche IPHEK-293T-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15366-1-AP bindet in WB, IP, IF, CoIP, ELISA RIPK2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RIPK2 fusion protein Ag0909
Vollständiger Name receptor-interacting serine-threonine kinase 2
Berechnetes Molekulargewicht 61 kDa
Beobachtetes Molekulargewicht 46-55 kDa, 61-72 kDa
GenBank-ZugangsnummerBC004553
Gene symbol RIPK2
Gene ID (NCBI) 8767
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.

Protokolle

Produktspezifische Protokolle
WB protocol for RIPK2 antibody 15366-1-APProtokoll herunterladen
IP protocol for RIPK2 antibody 15366-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
pigWB

Antioxidants (Basel)

M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide

Authors - Menghui Xu
humanWB

Obesity (Silver Spring)

Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance.

Authors - Yanping Wang
humanWB

J Pharmacol Exp Ther

Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.

Authors - Mohamed Salla
  • KD Validated
mouseWB

Front Cell Infect Microbiol

Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.

Authors - Chun-Xia Shi
mouseWB,IP

Immunol Lett

A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation.

Authors - Dong-Yi Cao
humanWB

Front Genet

Knockdown of RIPK2 Inhibits Proliferation and Migration, and Induces Apoptosis via the NF-κB Signaling Pathway in Gastric Cancer.

Authors - Qian Yang
  • KD Validated