RIC8A Monoklonaler Antikörper

RIC8A Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E3B11

Kat-Nr. : 66625-1-Ig

Synonyme

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66625-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66625-1-Ig (RIC8A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66625-1-Ig

  IP result of anti-RIC8A (IP:66625-1-Ig, 5ug; Detection:66625-1-Ig 1:2000) with HEK-293 cells lysate 4000 ug.

• 

  IHC staining of mouse brain using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66625-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66625-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66625-1-Ig bindet in WB, IP, IHC, IF, ELISA RIC8A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RIC8A fusion protein Ag1593
• Vollständiger Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC011821
• Gene symbol: RIC8A
• Gene ID (NCBI): 60626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.