RHOG-Specific Polyklonaler Antikörper

RHOG-Specific Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19671-1-AP

Synonyme

ARHG, RHOG, RHOG-Specific

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 19671-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19671-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19671-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 19671-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 19671-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 19671-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 19671-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19671-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, C6-Zellen, HeLa-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19671-1-AP bindet in WB, ELISA RHOG-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ras homolog gene family, member G (rho G)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: NM_001665
• Gene symbol: RHOG
• Gene ID (NCBI): 391
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.