RHOA Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

RHOA Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

CloneNo.

1B3D7

Rezensionen (1)

Kat-Nr. : 66733-1-Ig

Synonyme

ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

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Galerie der Validierungsdaten

• 

  WB analysis using 66733-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66733-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66733-1-Ig bindet in WB, IHC, IF, ELISA RHOA und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RHOA fusion protein Ag1141
• Vollständiger Name: ras homolog gene family, member A
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC005976
• Gene symbol: RHOA
• Gene ID (NCBI): 387
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

Publikationen

Species	Application	Title
human	IHC	Cell Death Differ; Mirtronic miR-4646-5p promotes gastric cancer metastasis by regulating ABHD16A and metabolite lysophosphatidylserines.; Authors - Liping Yang
human	WB	Clin Sci (Lond); The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.; Authors - Xueying Hu
human	WB	J Biol Chem; SARS-CoV-2 hijacks macropinocytosis to facilitate its entry and promote viral spike-mediated cell-to-cell fusion; Authors - Yu-Yuan Zhang ; KD Validated
human	WB	Front Mol Biosci; CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.; Authors - Fan Feng
human	WB	J Cancer; GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer.; Authors - Liu Jie
human	WB	Exp Cell Res; RALY may cause an aggressive biological behavior and a dismal prognosis in non-small-cell lung cancer.; Authors - Guanchu Song

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (12-22-2023)

Worked well for WB in Glioma cells at 1:1000 in BSA

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma