RHEB Monoklonaler Antikörper

RHEB Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F9H8

Kat-Nr. : 66714-1-Ig

Synonyme

GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2

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Galerie der Validierungsdaten

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  WB analysis of fetal human brain using 66714-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66714-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66714-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 66714-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of human pancreas cancer using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse pancreas using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human pancreas cancer using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse pancreas using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, DU 145-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IF: humanes Pankreaskarzinomgewebe, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66714-1-Ig bindet in WB, IF, ELISA RHEB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RHEB fusion protein Ag8887
• Vollständiger Name: Ras homolog enriched in brain
• Berechnetes Molekulargewicht: 184 aa, 20 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC016155
• Gene symbol: RHEB
• Gene ID (NCBI): 6009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.