RGS4 Monoklonaler Antikörper

RGS4 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1E2E2

Kat-Nr. : 66441-1-Ig

Synonyme

RGP4, RGS4, SCZD9

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Galerie der Validierungsdaten

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  WB analysis of Neuro-2a using 66441-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66441-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 66441-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66441-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66441-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66441-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, C6-Zellen, Raji-Zellen, SH-SY5Y-Zellen, U-251-Zellen, Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66441-1-Ig bindet in WB, IHC,ELISA RGS4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RGS4 fusion protein Ag6029
• Vollständiger Name: regulator of G-protein signaling 4
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC051869
• Gene symbol: RGS4
• Gene ID (NCBI): 5999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

Publikationen

Species	Application	Title
mouse	IHC	Hippocampus; NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.; Authors - Yan-Cong Wang