RGS3 Monoklonaler Antikörper

RGS3 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D6A3

Kat-Nr. : 66790-1-Ig

Synonyme

C2PA, FLJ20370, FLJ31516, FLJ90496, PDZ RGS3, RGP3, RGS3

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 66790-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66790-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66790-1-Ig bindet in WB, ELISA RGS3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RGS3 fusion protein Ag3177
• Vollständiger Name: regulator of G-protein signaling 3
• Berechnetes Molekulargewicht: 1198 aa, 132 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC018072
• Gene symbol: RGS3
• Gene ID (NCBI): 5998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGS3 belongs to a family of proteins which share the core regulator of G-protein signalling (RGS) domain and function as GTPase-activating proteins (GAPs) for the α subunit of heterotrimeric G-proteins. RGS3 has been reported to inhibit the Gai-and Gaq-mediated signaling pathways in the cardiovascular system, including those acting via the cardiovascular signaling molecules angiotensin II (Ang II), sphingosine 1-phosphate and endothelin-1. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear.