RGN/SMP30 Polyklonaler Antikörper

RGN/SMP30 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 17947-1-AP

Synonyme

RC, Regucalcin, RGN, RGN/SMP30, Senescence marker protein 30, SMP 30, SMP30

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Galerie der Validierungsdaten

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  WB analysis using 17947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 17947-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17947-1-AP

  IP result of anti-RGN/SMP30 (IP:17947-1-AP, 4ug; Detection:17947-1-AP 1:15000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of rat kidney using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50.

• 

  IHC staining of human liver using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 17947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Dünndarmgewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Rattennierengewebe, humanes Lebergewebe, humanes Leberzirrhosegewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17947-1-AP bindet in WB, IP, IHC, IF, ELISA RGN/SMP30 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGN/SMP30 fusion protein Ag12423
• Vollständiger Name: regucalcin (senescence marker protein-30)
• Berechnetes Molekulargewicht: 299 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 25 kDa
• GenBank-Zugangsnummer: BC073173
• Gene symbol: RGN
• Gene ID (NCBI): 9104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Regucalcin (RGN), also known as SMP30, is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. SMP30 is preferentially localized in hepatocytes and renal proximal tubular epithelial cells. It is known to be related to aging, hepatocyte proliferation and tumorigenesis. It may be useful for HCC serologic screening, especially for the patients with AFP Negative. RGN encodes two isoforms: 33 kDa and 25 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Transl Lung Cancer Res; Senescence marker protein 30 inhibits tumor growth by reducing HDAC4 expression in non-small cell lung cancer.; Authors - Changjian Shao
human,mouse	WB	Ecotoxicol Environ Saf; Hexavalent chromium triggers hepatocytes premature senescence via the GATA4/NF-κB signaling pathway mediated by the DNA damage response.; Authors - Yu Ma
human	WB	Ecotoxicol Environ Saf; Identification and functional analysis of senescence-associated secretory phenotype of premature senescent hepatocytes induced by hexavalent chromium.; Authors - Yu Ma
bovine	WB	J Endocrinol; Hepatic thyroid signaling of heat-stressed late pregnant and early lactating cows.; Authors - Joachim Weitzel
mouse	WB	Int Immunopharmacol; Senescence marker protein 30 (SMP30) protects against high glucose-induced apoptosis, oxidative stress and inflammatory response in retinal ganglion cells by enhancing Nrf2 activation via regulation of Akt/GSK-3β pathway; Authors - Le Zhang
mouse	WB	Toxicol Lett; Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo.; Authors - Zhao Xiaoming X