RFWD3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RFWD3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19893-1-AP

Synonyme

RFWD3, RING finger protein 201, RNF201

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse lung using 19893-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19893-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human testis using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human colon using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 19893-1-AP

  Immunofluorescent analysis of 293 cells, using RFWD3 antibody 19893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 19893-1-AP

  Immunofluorescent analysis of Hela cells, using RFWD3 antibody 19893-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

19893-1-AP bindet in WB, IHC, IF, ELISA RFWD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFWD3 fusion protein Ag13728
• Vollständiger Name: ring finger and WD repeat domain 3
• Berechnetes Molekulargewicht: 774 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC002574
• Gene symbol: RFWD3
• Gene ID (NCBI): 55159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; DOCK7 protects against replication stress by promoting RPA stability on chromatin.; Authors - Ming Gao
human	WB,IF	Front Oncol; The Valproate Mediates Radio-Bidirectional Regulation Through RFWD3-Dependent Ubiquitination on Rad51.; Authors - Guochao Liu ; KD Validated
human	IHC	Front Med; Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer.; Authors - Yanfei Zhang
human	IHC	Histol Histopathol; RFWD3 acts as a tumor promotor in the development and progression of bladder cancer; Authors - Peng Jiang