REPS2 Polyklonaler Antikörper

REPS2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 20699-1-AP

Synonyme

Partner of RalBP1, POB1, RalBP1 interacting protein 2, REPS2

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20699-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 20699-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 20699-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20699-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20699-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20699-1-AP

  Immunofluorescent analysis of HepG2 cells, using REPS2 antibody 20699-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hodengewebe, LNCaP-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

20699-1-AP bindet in WB, IF, ELISA REPS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RALBP1 associated Eps domain containing 2
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa, 55 kDa, 30 kDa
• GenBank-Zugangsnummer: NM_004726
• Gene symbol: REPS2
• Gene ID (NCBI): 9185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REPS2, also named as POB1, is involved in growth factor signaling through its influence on the Ral signaling pathway. The antibody is specific to REPS2.

Publikationen

Species	Application	Title
human	IF	Cell Signal; REPS2 downregulation facilitates FGF-induced adhesion and migration in human lens epithelial cells through FAK/Cdc42 signaling and contributes to posterior capsule opacification.; Authors - Chan Wen