RECQL4 Polyklonaler Antikörper

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• KD/KO Validated

RECQL4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17008-1-AP

Synonyme

ATP dependent DNA helicase Q4, DNA helicase, RecQ like type 4, RecQ protein like 4, RECQ4, RECQL4, RTS

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Galerie der Validierungsdaten

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  WB analysis using 17008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17008-1-AP

  IP result of anti-RECQL4(IP:17008-1-AP, 4ug; Detection:17008-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of human gliomas using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17008-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RECQL4 antibody (17008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

17008-1-AP bindet in WB, IP, IHC, IF, ELISA RECQL4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RECQL4 fusion protein Ag10707
• Vollständiger Name: RecQ protein-like 4
• Berechnetes Molekulargewicht: 1208 aa, 133 kDa
• Beobachtetes Molekulargewicht: 145-150 kDa
• GenBank-Zugangsnummer: BC013277
• Gene symbol: RECQL4
• Gene ID (NCBI): 9401
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

Publikationen

Species	Application	Title
human	WB,IP	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IHC	Cancer Biol Med; RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer.; Authors - Guosheng Lyu ; KD Validated
human	WB	FASEB J; UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair.; Authors - Qiuling Huang
human	WB	J Biol Chem; The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.; Authors - Jiewei Wang
human	IF	Sci Rep; PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.; Authors - Sebastian Veith