RDH10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RDH10 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Rezensionen (2)

Kat-Nr. : 14644-1-AP

Synonyme

RDH10, Retinol dehydrogenase 10, SDR16C4

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Galerie der Validierungsdaten

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  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14644-1-AP

  IP result of anti-RDH10(IP:14644-1-AP, 4ug; Detection:14644-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of mouse kidney using 14644-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14644-1-AP (RDH10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14644-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14644-1-AP (RDH10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, Mauslebergewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

14644-1-AP bindet in WB, IP, IHC, IF, ELISA RDH10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Insekt
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RDH10 fusion protein Ag6237
• Vollständiger Name: retinol dehydrogenase 10 (all-trans)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC067131
• Gene symbol: RDH10
• Gene ID (NCBI): 157506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).

Publikationen

Species	Application	Title
human	IF	Nat Commun; Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue.; Authors - Atsushi Kuwahara
mouse	WB	Proc Natl Acad Sci U S A; Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct.; Authors - Tadaaki Nakajima
mouse		Curr Biol; Multiple Isoforms of Nesprin1 Are Integral Components of Ciliary Rootlets.; Authors - Chloe Potter
mouse	WB	Cell Prolif; Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120; Authors - Jun Jing
mouse	IF	Cell Rep; Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.; Authors - Tony DeFalco
human	IF	Stem Cell Reports; Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.; Authors - Albert Lowe

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Claudio (Verified Customer) (06-29-2020)

Antibody did not show specific staining/signal. Antigen retrieval pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: PFA-fixed paraffin-embedded mouse sections

FH

Daniel (Verified Customer) (02-12-2019)

Validated with CRISPR/Cas9 knockout cell lines

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO)

RDH10 Antibody Western Blot, validation (1:1000 dilution) in Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO) (Cat no:14644-1-AP)