RCC1 Polyklonaler Antikörper

RCC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 22142-1-AP

Synonyme

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

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Galerie der Validierungsdaten

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  WB analysis of A431 using 22142-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22142-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22142-1-AP

  IP Result of anti-RCC1 (IP:22142-1-AP, 3ug; Detection:22142-1-AP 1:1000) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human testis using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 22142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 22142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 22142-1-AP

  Immunofluorescent analysis of A431 cells using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

22142-1-AP bindet in WB, IP, IHC, IF, ELISA RCC1 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCC1 fusion protein Ag17569
• Vollständiger Name: regulator of chromosome condensation 1
• Berechnetes Molekulargewicht: 421 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC010067
• Gene symbol: RCC1
• Gene ID (NCBI): 1104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1.

Publikationen

Species	Application	Title
human	IF	Curr Biol; Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development.; Authors - Diana Vargas-Hurtado
mouse	WB	Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song
human	WB	J Med Chem; Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.; Authors - Brianna D Mackie
human	IHC	Cell Death Dis; Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells.; Authors - Lijun Qiao
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	J Cell Mol Med; SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing; Authors - Xi Chen