RCAS1 Monoklonaler Antikörper

RCAS1 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

4H8A12

Kat-Nr. : 66170-1-Ig

Synonyme

EB9, EBAG9, PDAF, RCAS1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66170-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66170-1-Ig (RCAS1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human lung cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human lung cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IF Staining of human breast cancer using 66170-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RCAS1 antibody (66170-1-Ig, Clone: 4H8A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 66170-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RCAS1 antibody (66170-1-Ig, Clone: 4H8A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  FC experiment of Jurkat using 66170-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human RCAS1 (66170-1-Ig, Clone:4H8A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

66170-1-Ig bindet in WB, IHC, IF, FC, ELISA RCAS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RCAS1 fusion protein Ag2905
• Vollständiger Name: estrogen receptor binding site associated, antigen, 9
• Berechnetes Molekulargewicht: 213 aa, 24 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC017729
• Gene symbol: EBAG9
• Gene ID (NCBI): 9166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

Publikationen

Species	Application	Title
mouse	WB,IF	Mol Med Rep; RCAS1 increases cell morphological changes in murine fibroblasts by reducing p38 phosphorylation; Authors - Takuya Nishinakagawa