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RBP5 Monoklonaler Antikörper

RBP5 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G7E2

Kat-Nr. : CL488-68126

Synonyme

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RBP5 Monoclonal anti (CL488-68126)

    IF Staining of HepG2 using CL488-68126

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated RBP5 Monoclonal anti (CL488-68126)

    IF Staining of A431 using CL488-68126

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68126 bindet in IF RBP5 und zeigt Reaktivität mit Hausschwein, human

Getestete Reaktivität Hausschwein, human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RBP5 fusion protein Ag24562
Vollständiger Name retinol binding protein 5, cellular
Berechnetes Molekulargewicht 135 aa, 16 kDa
Beobachtetes Molekulargewicht 16-18 kDa
GenBank-ZugangsnummerBC029355
Gene symbol RBP5
Gene ID (NCBI) 83758
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 RBP5 antibody CL488-68126Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen