RBP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 22683-1-AP

Synonyme

CRABP I, CRBP, CRBP I, CRBP1, CRBPI, RBP1, RBPC, Retinol binding protein 1

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Galerie der Validierungsdaten

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  WB analysis using 22683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 22683-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22683-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 22683-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 22683-1-AP (RBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen, humanes Hirngewebe, humanes Lebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

22683-1-AP bindet in WB, IHC, IF, ELISA RBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBP1 fusion protein Ag18572
• Vollständiger Name: retinol binding protein 1, cellular
• Berechnetes Molekulargewicht: 135 aa, 16 kDa
• Beobachtetes Molekulargewicht: 14-21 kDa
• GenBank-Zugangsnummer: BC121052
• Gene symbol: RBP1
• Gene ID (NCBI): 5947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu
rat	IHC	Brain Sci; Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus.; Authors - Tianjia Li
mouse	WB	J Food Biochem; Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.; Authors - Wei Li
human	WB,IHC,IF	Cell Death Dis; The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.; Authors - Ling Gao ; KD Validated
human	WB	FASEB J; The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation; Authors - Dan-Dan Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Melissa (Verified Customer) (06-13-2022)

We have used the antibody and it worked out

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1
• Cell Tissue Type: Endothelial cells