RBM39 Polyklonaler Antikörper

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• KD/KO Validated

RBM39 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21339-1-AP

Synonyme

CAPER, CAPERalpha, CC1.3, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21339-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21339-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 21339-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21339-1-AP (RBM39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21339-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21339-1-AP (RBM39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 21339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 21339-1-AP (RBM39 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, humanes Skelettmuskelgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Produktinformation

21339-1-AP bindet in WB, RIP, IHC, IF, ELISA RBM39 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM39 fusion protein Ag15584
• Vollständiger Name: RNA binding motif protein 39
• Berechnetes Molekulargewicht: 530 aa, 59 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC141835
• Gene symbol: RBM39
• Gene ID (NCBI): 9584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

Publikationen

Species	Application	Title
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	Haematologica; Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.; Authors - Jia Tong
human	WB	J Proteome Res; Proximity Labeling, Quantitative Proteomics, and Biochemical Studies Revealed the Molecular Mechanism for the Inhibitory Effect of Indisulam on the Proliferation of Gastric Cancer Cells.; Authors - Jiaqi Lu ; KD Validated
human	WB	Cell Rep; Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.; Authors - Tabitha C Ting
human	IHC,RIP	Cell Death Discov; The cancer-testis lncRNA LINC01977 promotes HCC progression by interacting with RBM39 to prevent Notch2 ubiquitination; Authors - Anliang Xia