RBM15B Monoklonaler Antikörper

RBM15B Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1C2C11

Kat-Nr. : 67506-1-Ig

Synonyme

HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B

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Galerie der Validierungsdaten

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  WB analysis using 67506-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 67506-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 67506-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67506-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67506-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67506-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67506-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67506-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67506-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67506-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, COLO 320-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67506-1-Ig bindet in WB, IHC, ELISA RBM15B und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBM15B fusion protein Ag17853
• Vollständiger Name: RNA binding motif protein 15B
• Berechnetes Molekulargewicht: 890 aa, 97 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC001367
• Gene symbol: RBM15B
• Gene ID (NCBI): 29890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Wilms' Tumor 1-Associating Protein complex regulates alternative splicing and polyadenylation at potential G-quadruplex-forming splice site sequences.; Authors - Keiko Horiuchi
human	WB	Nat Commun; CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes; Authors - Yanfen Zheng