RBM15B Polyklonaler Antikörper

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• KD/KO Validated

RBM15B Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 22249-1-AP

Synonyme

HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 22249-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22249-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22249-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22249-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22249-1-AP

  IP Result of anti-RBM15B (IP:22249-1-AP, 4ug; Detection:22249-1-AP 1:500) with HeLa cells lysate 2500ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

22249-1-AP bindet in WB, RIP, IP, IHC, CoIP, ELISA RBM15B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM15B fusion protein Ag17744
• Vollständiger Name: RNA binding motif protein 15B
• Berechnetes Molekulargewicht: 890 aa, 97 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa, 70 kDa
• GenBank-Zugangsnummer: BC001367
• Gene symbol: RBM15B
• Gene ID (NCBI): 29890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa), thus this antibody can recognize full length of RBM15B(~100kDa) and splice-one(70kDa)

Publikationen

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu ; KO Validated
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	RIP	Elife; SNORD90 induces glutamatergic signaling following treatment with monoaminergic antidepressants; Authors - Rixing Lin
human	WB	eNeuro; Altered Expression of the m6A Methyltransferase METTL3 in Alzheimer's Disease.; Authors - He Huang
human	IHC	Front Genet; m6A Regulatory Gene-Mediated Methylation Modification in Glioma Survival Prediction.; Authors - Guiyun Zhang
human	IHC	Med Sci Monit; Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.; Authors - Yingying Zhang