RBM15 Monoklonaler Antikörper

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• KD/KO Validated

RBM15 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

4A1A4

Kat-Nr. : 66059-1-Ig

Synonyme

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

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Galerie der Validierungsdaten

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  WB analysis using 66059-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK293 cells using 66059-1-Ig

  WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells.

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  WB analysis of HeLa using 66059-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66059-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66059-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66059-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66059-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66059-1-Ig

  IP Result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug.

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  IHC staining of human breast cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66059-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Herzgewebe, humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

66059-1-Ig bindet in WB, IP, IHC, IF, ELISA RBM15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBM15 fusion protein Ag17970
• Vollständiger Name: RNA binding motif protein 15
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC006397
• Gene symbol: RBM15
• Gene ID (NCBI): 64783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Commun; Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.; Authors - Yuxian Guo
human	WB	Elife; Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.; Authors - Li Zhang
	WB	EMBO Rep; The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation.; Authors - Ngoc-Tung Tran
human	IHC	J Gene Med; The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.; Authors - Yujie Li