RBL2 Polyklonaler Antikörper

RBL2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 27251-1-AP

Synonyme

P130, pRb2, Rb2, RBL2, RBR 2, retinoblastoma like 2 (p130), Retinoblastoma like protein 2

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Galerie der Validierungsdaten

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  WB analysis of A549 using 27251-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27251-1-AP (RBL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 27251-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27251-1-AP (RBL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27251-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27251-1-AP (RBL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

27251-1-AP bindet in WB, ELISA RBL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBL2 fusion protein Ag25087
• Vollständiger Name: retinoblastoma-like 2 (p130)
• Berechnetes Molekulargewicht: 130 kDa
• Beobachtetes Molekulargewicht: 128 kDa
• GenBank-Zugangsnummer: BC034490
• Gene symbol: RBL2
• Gene ID (NCBI): 5934
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBL2, also named as Retinoblastoma-like protein 2 or p130, is a 1130 amino acid protein, which Belongs to the retinoblastoma protein (RB) family. RBL2 is a Key regulator of entry into cell division and is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. RBL2 probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 may act as a tumor suppressor.

Publikationen

Species	Application	Title
mouse	WB	Biochem Pharmacol; Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms.; Authors - Ji Young Kim
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen
mouse	WB	Life Sci; Early-senescent bone marrow mesenchymal stem cells promote C2C12 cell myogenic differentiation by preventing the nuclear translocation of FOXO3.; Authors - Ji Che
human	WB	Nat Commun; An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis; Authors - Muqing Cao ; KD Validated