RBAP48 Monoklonaler Antikörper

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• KD/KO Validated

RBAP48 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5C4D6

Kat-Nr. : 66060-1-Ig

Synonyme

Histone binding protein RBBP4, RBAP48

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Galerie der Validierungsdaten

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  WB analysis using 66060-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66060-1-Ig

  WB result of RBAP48 antibody (66060-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.

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  WB analysis of HeLa using 66060-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66060-1-Ig(RBAP48 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66060-1-Ig(RBAP48 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66060-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66060-1-Ig (RBAP48 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66060-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human RBAP48 (66060-1-Ig, Clone:5C4D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, L02-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66060-1-Ig bindet in WB, IHC, IF, FC, ELISA RBAP48 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBAP48 fusion protein Ag6196
• Vollständiger Name: retinoblastoma binding protein 4
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC053904
• Gene symbol: RBBP4
• Gene ID (NCBI): 5928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

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