RBAP48 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBAP48 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 20364-1-AP

Synonyme

CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4

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Galerie der Validierungsdaten

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  WB analysis using 20364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20364-1-AP

  WB result of RBAP48 antibody (20364-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.

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  WB analysis of human brain using 20364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20364-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20364-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20364-1-AP

  IP Result of anti-RBAP48 (IP:20364-1-AP, 4ug; Detection:20364-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBAP48 antibody (20364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen, L02-Zellen, Maushirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Produktinformation

20364-1-AP bindet in WB, IP, IHC, IF, ELISA RBAP48 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: retinoblastoma binding protein 4
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: NM_005610
• Gene symbol: RBBP4
• Gene ID (NCBI): 5928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB, IHC	J Clin Invest; ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.; Authors - Pingping Zhu
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
	WB	Carcinogenesis; circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression.; Authors - Wei Zhan
human,mouse	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
human	WB	Chembiochem; Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells.; Authors - Fang-Fang Zhuo ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katherine (Verified Customer) (09-25-2023)

Nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T