RBAP48 Polyklonaler Antikörper

RBAP48 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14557-1-AP

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14557-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14557-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14557-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14557-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14557-1-AP bindet in WB, IHC, IF,ELISA RBAP48 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBAP48 fusion protein Ag6110
• Vollständiger Name: retinoblastoma binding protein 4
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 40 kDa, 48-53 kDa
• GenBank-Zugangsnummer: BC053904
• Gene symbol: RBBP4
• Gene ID (NCBI): 5928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.