RBAP48 Monoklonaler Antikörper

RBAP48 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

5C4D6

Kat-Nr. : CL594-66060

Synonyme

CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4

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Galerie der Validierungsdaten

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  IF Staining of A549 using CL594-66060

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594-conjugated RBAP48 antibody (CL594-66060, Clone: 5C4D6 ) at dilution of 1:200, CL488-Phalloidin (green).

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  FC experiment of HepG2 using CL594-66060

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human RBAP48 (CL594-66060, Clone:5C4D6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of A549 using CL594-66060

  1X10^6 A549 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human RBAP48 (CL594-66060, Clone:5C4D6) (red), or 0.2 ug Control Antibody (CL594-66360-3, Clone: ) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen, A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66060 bindet in IF, FC (Intra) RBAP48 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBAP48 fusion protein Ag6196
• Vollständiger Name: retinoblastoma binding protein 4
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC053904
• Gene symbol: RBBP4
• Gene ID (NCBI): 5928
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011