RASGRP3 Monoklonaler Antikörper

RASGRP3 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10G4

Rezensionen (1)

Kat-Nr. : 66650-1-Ig

Synonyme

GRP3, KIAA0846, Ras guanyl releasing protein 3, RASGRP3

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Galerie der Validierungsdaten

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  WB analysis using 66650-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66650-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66650-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 66650-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RASGRP3 antibody (66650-1-Ig, Clone: 1A10G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HEK-293-Zellen, Jurkat-Zellen, Ramos-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66650-1-Ig bindet in WB, IF, ELISA RASGRP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RASGRP3 fusion protein Ag3810
• Vollständiger Name: RAS guanyl releasing protein 3 (calcium and DAG-regulated)
• Berechnetes Molekulargewicht: 690 aa, 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC027849
• Gene symbol: RASGRP3
• Gene ID (NCBI): 25780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt’s lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,