RASGRP2 Polyklonaler Antikörper

RASGRP2 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30189-1-AP

Synonyme

CALDAG GEFI, Cdc25 like protein, CDC25L, F25B3.3 kinase like protein, hCDC25L, MCG7, RAS guanyl releasing protein 2, RASGRP2

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Galerie der Validierungsdaten

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  WB analysis using 30189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30189-1-AP (RASGRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 30189-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human RASGRP2 (30189-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen, Raji-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30189-1-AP bindet in WB, FC, ELISA RASGRP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RASGRP2 fusion protein Ag32762
• Vollständiger Name: RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• Berechnetes Molekulargewicht: 69kd
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: NM_153819
• Gene symbol: RASGRP2
• Gene ID (NCBI): 10235
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.